Questions, answered
in writing

No — Bacillus F is a postbiotic. We deliver the stable, well-characterized metabolites of the Bacillus F organism, not live bacteria. That distinction drives everything about how the product behaves: composition stays consistent from batch to batch, the bottle needs no refrigeration, and nothing depends on organisms surviving stomach acid and establishing themselves in the gut. A probiotic is a living population with a shelf life measured in viable counts. A postbiotic is a finished chemical preparation, so what leaves our fermentation run is what reaches you. The patent family and the comet-assay imaging behind that preparation are published in full on the Science page, so you can read the evidence rather than take our description of it on trust.

A probiotic delivers live organisms that have to survive stomach acid, reach the gut intact and then establish themselves. Each of those is a failure point, which is why live-culture products carry refrigeration requirements, expiry-linked viability counts, and a gap between the number printed on the label and the number that actually arrives. A postbiotic skips the organism entirely and delivers the finished metabolites — the compounds the bacterium produces — directly. There is nothing alive to keep alive. What is in the bottle on day ninety is what was in it on day one, because the preparation is chemically stable rather than biologically active. That is also why there is no viability count on the label: there is nothing alive to count.

A sublingual applicator pen. The outer sleeve is white with a liquid-level window so you can see how much protocol is left, and inside it sits a clear cartridge with a measured-dose tip. The daily dose is 1 ml, dripped under the tongue in the morning, held there briefly and then swallowed. One cartridge is one 30-day protocol, which is also the unit we sell and price — $169 per bottle. There are no capsules, no powder to weigh and no mixing step. We chose the sublingual pen because the dose is measured by the applicator rather than by the user, which keeps a 30-day protocol consistent from the first day to the last. Photographs of the pen, the cartridge and the dosing tip are on the product pages.

No. Store the pen at room temperature, away from direct light, exactly as it arrives. Because nothing in the preparation is alive, there is no viability to preserve — the reason live-culture probiotics need a cold chain is that their organisms die at room temperature, and a postbiotic has no organisms to lose. This matters practically as well as chemically: the product ships worldwide in monthly batches and spends time in transit and in customs, and no part of that journey depends on a refrigerated courier. It also means you can travel with the pen. Keep it out of direct sunlight and away from a hot car dashboard, treat it like any other room-temperature supplement, and the batch date and best-before printed on the label are the only dates you need to watch.

Each bottle carries its batch date and best-before date on the label, so the answer for your specific pen is printed on the pen rather than generalised here. Because the preparation is chemically stable rather than biologically active, its profile does not drift the way a live-culture product does: there is no declining viable count between manufacture and the date on the label, and no meaningful difference between a bottle opened on day one of its window and one opened later. Store it at room temperature away from direct light, use one cartridge as one 30-day protocol, and check the label date if a pen has been sitting in a drawer. If a batch date is unclear or the label is damaged in transit, write to info@bacillusf.com and we will confirm the batch from our records.

The strain was recovered from Siberian permafrost cores at Mammoth Mountain in Yakutia and revived in culture in 2009. Its discovery, its genome sequencing and its patent family — WO2012060729 plus five national patents — are documented, and the scans are published on our Science page rather than summarised. The dating figure refers to the sediment layer the sample came from, established by geocryological methods, not to a measurement of the organism itself; that is a distinction we keep making because the difference matters. So the honest version is this: the permafrost recovery, the culture date, the genome deposit and the patent family are all documented and readable. What that history does not do is establish an effect in humans, and we do not present it as if it did.

Single-cell gel electrophoresis. Cells are embedded in agarose, lysed so that only their DNA remains, then placed in an electric field. Intact DNA is too large to migrate and stays in place; fragmented DNA moves and forms a visible "comet tail" whose length and intensity quantify the damage present in that individual cell. Because the readout is per cell rather than averaged across a sample, it is one of the standard methods for DNA-integrity work, and it is why our reference imaging on the Science page is comet-assay imaging rather than a stylised illustration. We publish the before and after frames so the method is visible rather than described. It is laboratory work, not a human trial, and we label it that way everywhere it appears.

No. We do not claim any, and you should be sceptical of any supplement brand in this category that claims trials without publishing the registration number and the results. Saying so costs us a marketing line, and we would rather lose the line than blur the difference between laboratory work and clinical evidence. What does exist is documented and published in full on the Science page: the recovered and characterised strain, the patent family including WO2012060729, the genome deposit, and laboratory comet-assay work with the imaging attached. That is the whole evidence base, presented as what it is. If human trial data ever exists, it will appear on the Science page with its registration, not in a headline.

One measured dose — 1 ml from the pen — dripped under the tongue in the morning. Hold it there briefly before swallowing, which is the whole of the sublingual step; there is nothing to mix, weigh or time around meals. One cartridge covers a 30-day protocol, and the liquid-level window on the sleeve shows you where you are in it. We suggest taking it at the same point in the morning each day, not because a missed hour undoes anything, but because a fixed cue is what makes a 90-day observation window actually run for 90 days. Store the pen at room temperature between doses. If you miss a day, take the next dose as normal rather than doubling it — the protocol is a daily cadence, not a cumulative total.

Yes. These are three separate mechanisms rather than three versions of the same thing: NMN targets NAD+ levels, spermidine targets autophagy, and Bacillus F works on oxidative-stress defence and cellular resilience. Because they do not overlap, many customers run all three together, and the head-to-head comparisons in Learn set out what each one is and what documentation stands behind it — including where the evidence for each is thinner than the marketing around it. We do not sell a stack, we do not claim a synergy between them, and none of these comparisons is medical advice. If you already take prescription medication or manage a health condition, the conversation about combining anything belongs with your physician, and that includes this product.

Subjective effects vary from person to person, and we recommend judging by numbers rather than by feelings — a supplement in this category is exactly the sort of thing that day-to-day mood, sleep and workload will happily take credit for. So take a baseline first: a biological-age panel or an oxidative-stress panel, measured before you start. Then run 90 days, then re-test on the same panel. The protocol is designed around that cadence, which is why one cartridge is 30 days and why we recommend three of them before drawing any conclusion. We make no claim about what your re-test will show. What we can say is that a measurement taken before and after is the only version of this question that produces an answer you can check.

None are reported for the preparation. That said, the honest framing matters: we are a wellness supplement, not a medicine, and a clean safety record is not a study of interactions with your prescriptions. So if you are pregnant, nursing, taking prescription medication, or managing a health condition of any kind, talk to your physician before adding anything to your routine — including this. We would rather you ask and skip it than take it on our reassurance. If your physician wants to see the evidence before answering, the patent scans and the comet-assay imaging are on the Science page.

Anyone under 18, and anyone whose physician advises against it. Beyond that, the same caution applies to anyone who is pregnant, nursing, taking prescription medication or managing a diagnosed condition: ask your physician first, and treat a no as the answer. We do not sell Bacillus F as a treatment for any condition, we make no clinical or disease claims anywhere on this site, and it should never be used in place of medical care or as a reason to delay it. That is not legal boilerplate at the bottom of a page — it is the same position we take in every answer here, and the reason the Science page publishes the documentation we do have instead of the claims we do not.

$169 per 30-day protocol — one bottle, one cartridge, thirty daily 1 ml doses. That is the whole price list: there is no subscription, no tiered membership, and no bulk discount published on the site. Shipping is not included in that figure because it depends on your destination; it is confirmed per country in the written quote you receive when you request access, so you see the full amount before anything is invoiced. Because we recommend a 90-day observation window with a baseline test and a re-test, most customers are budgeting three bottles rather than one, and we would rather say that plainly here than have it emerge at the third order. Practitioners, clinics and distributors are priced separately — see the wholesale page.

Access is by request, and the sequence is deliberate. You write to us, we confirm availability for your country within 48 hours and send a written quote including shipping, and only then do we invoice. Nothing is charged before that confirmation. The reason is supply: fermentation runs are small and quality-gated, they are released in monthly batches, and we would rather tell you that a batch is already committed than take payment against stock we cannot ship. A checkout button would let us sell what does not exist yet. It also means every order carries a written trail — your request, our confirmation, the quote — which is the same standard we hold ourselves to for the documentation on the Science page.

By design. Every request goes through the form to info@bacillusf.com, so nothing is lost between a conversation and an order, and every answer we give is written, referenced and reviewable afterwards. For a product where the honest answer to several questions is "here is the document, and here is what it does not cover", a phone call is the wrong medium — it produces reassurance that neither side can check later. Written replies come within 48 hours, they quote the same documentation published on the Science page, and you keep a copy. If you need to discuss wholesale, clinic or distribution terms, that also starts in writing on the wholesale page and moves to a call once there is something specific to talk about.

We ship worldwide in monthly batches, with localised support for the US, UK, Canada, Japan, Germany, France and Spain — those are the markets where the site, the documentation and the replies exist in the local language. Shipping itself is not limited to that list. What varies is import rules: supplement classification, permitted ingredients and customs handling differ by country and change without notice, so publishing a definitive list of destinations would mean publishing something that goes stale between batches. Instead we confirm your specific destination as part of the 48-hour reply, together with the shipping cost for that country, before anything is invoiced. If your country turns out to be a problem, you will hear that in the reply rather than after payment.

Yes. Practitioners, clinics and distributors are handled separately from individual orders, with their own pricing, minimum quantities and documentation supplied directly rather than pointed at. Start on the wholesale page, which sets out what we need to know: the market you serve, the volume you expect per month, and whether you are dispensing to clients or reselling. Because fermentation runs are small and released in monthly batches, wholesale allocation is planned a batch ahead, so the first reply is usually about timing as much as price. As with individual orders, the process runs in writing through info@bacillusf.com and you receive a written quote before any invoice.